Genetic and biological factors influencing host response to porcine reproductive and respiratory syndrome virus in growing pigs
نویسندگان
چکیده
Porcine Reproductive and Respiratory Syndrome (PRRS) is a disease of major importance in the swine industry due to its economic impact and its negative effects on animal welfare. PRRS has been estimated to cost the US swine industry alone $664 million per year and impacts all stages of swine production. Current efforts to contain PRRS include biosecurity and vaccination strategies but these have not been widely successful, partly due to large genetic and antigenic variation observed between different PRRS virus (PRRSV) strains. The PRRS Host Genetics Consortium was created with the goal of increasing understanding of the host genetic response to PRRSV infection and evaluating the potential of genetic selection of pigs that are more resistant to PRRS. Approximately 200 commercial crossbred pigs in each of 15 trials were experimentally infected with either the NVSL-97-7895 (NVSL) or KS-2006-78109 (KS06) isolate of PRRSV at approximately 28 days of age. Serum was collected at 0, 4, 7, 11, 14, 21, 28, 35 and 42 days post infection (dpi). Weights were collected at 0 dpi and weekly thereafter. Tonsils and ear samples for genotyping were collected at 42 dpi. Serum viremia and tonsil virus levels were measured using a semi-quantitative PCR assay. The levels of serum PRRSV N protein specific immunoglobulin G was quantified at 42 dpi using fluorescent microsphere immunoassay, and measurements were reported as a sample to positive ratio (S:P). Monophasic or biphasic Wood’s curves were fitted to serum viremia records, and the parameters for these curves were used to derive serum curve characteristics for each animal. Additionally, 2 order Legendre polynomials were fitted to weight measurements. Serum viremia and weight curve fittings allowed daily serum viremia and daily weights to be interpolated to
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تاریخ انتشار 2016